Application

Anti-PEG, clone 6.3, Cat. No. MABS1966, is a mouse monoclonal antibody that detects PEG-conjugated proteins and it hs been tested for use in ELISA.

Research CategorySignaling

ELISA Analysis: A representative lot detected PEG in ELISA applications (Tung, H.Y., et. al. (2015). Mol Cancer Ther. 14(6):1317-26).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. Clone 6.3 displays high affinity and binds the PEG backbone and can be employed as a detection antibody for the quantification of PEG. It binds to a smaller epitope of the PEG backbone when compared to other antibodies. Since it binds to a smaller PEG epitope (~750 Da), it is not compatible with detergents that contain an ethylene oxide backbone, such as Tween 20. Instead 0.05% CHAPS is a more suitable detergent when using this antibody.

Immunogen

PEG-conjugated to E.coli beta-glucuronidase.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein A purified

Purified mouse monoclonal antibody IgG1 in PBS with 0.04% sodium azide.

Format: Purified

Quality

Evaluated by ELISA in Immobilized PEG (10K-PEG, 3K-PEG, and 2K-mPEG).

ELISA Analysis: 5 µg/mL of Immobilized PEG (10K-PEG, 3K-PEG, and 2K-mPEG) were detected with this antibody.

Specificity

Clone 6.3 specifically detects proteins conjugated with polyethylene glycol (PEG).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.